High Performance Liquid Chromatography Injection in Shugan baicalin and Geniposide Content.docVIP

 PAGE \* MERGEFORMAT 9 High Performance Liquid Chromatography Injection in Shugan baicalin and Geniposide Content Keywords: high-performance Abstract: Objective: To establish a high-performance liquid chromatography in Shugan Injection baicalin and Geniposide content. Methods: AtlantisTM dC18 column, 4.6 × 150mm, 5μ; baicalin mobile phase was 0.01mol / L phosphate buffer (pH2.8)-methanol (60:40), flow rate 1ml/min, detection wavelength 280nm; Gardenia glycosides mobile phase was methanol water glacial acetic acid (20:80:0.5), flow rate 1ml/min, detection wavelength 238nm. Results: baicalin and gardenoside linear in the range of 30 ~ 100μg/ml, 30 ~ 150μg/ml, the average recoveries were 99.68%, 99.66%. Conclusion: The method is simple, rapid, accurate and suitable for Shugan Injection quality control. Keywords:: Shugan Injection; Determination; baicalin; geniposide Shugan Injection by capillaris, gardenia, Huang Qin, Isatidis and Ganoderma lucidum extracts made of sterile aqueous solution, attending Qingrejiedu, Lishi back yellow, Qi righting, nourish the liver Liver is an effective treatment for all kinds of viral hepatitis traditional Chinese medicine preparations, the main active ingredient of baicalin and gardenoside. There were determined by high performance liquid chromatography, or Geniposide baicalin content of the reports [1,2], but the sample column operation cycle is longer, in more than 15min. This study established in Shugan Injection baicalin and geniposide high performance liquid chromatography assay method with rapid, sensitive, simple, specific and strong characteristics of the active ingredient may be better to eliminate the interference between the . An instrument and reagent Waters High Performance Liquid Chromatography (including the 600 quaternary gradient pump, on-line Degasser, column temperature box, 996 diode array detector, Empower chromatography workstation), METTLER AE240 electronic analytical b