High Performance Liquid Chromatography in Oldenlandia diffusa 3,4 - 2-hydroxybenzoate content.docVIP

 PAGE \* MERGEFORMAT 9 High Performance Liquid Chromatography in Oldenlandia diffusa 3,4 - 2-hydroxybenzoate content Author: Ma Lin LI Jun-ming Chen Yuqing Li Yan GUO Xing-jie [Abstract] Objective To determine the Oldenlandia diffusa in 3,4 - 2-hydroxybenzoate content of high performance liquid chromatography (HPLC) method. Methods ODS column (200 mm * 4.6 mm, 5 μ m), with methanol -0.05% phosphoric acid solution (15:85, V / V) as mobile phase, detection wavelength at 254 nm. 3,4 - 2-hydroxybenzoate concentration of 2.5 ~ 50 μ g * ml-1 within the range of peak area showed good linear relationship (r = 0.999 4); sample recovery was 100.4% (n = 9); the results of good precision and reproducibility, RSD does not exceed 2.6%. Conclusion The method is simple, accurate, and can be used as quality control of medicinal herbs Oldenlandia diffusa a quantification. [Keywords:] high performance liquid chromatography Hedyotis diffusa 3,4 - 2-hydroxybenzoate Rubiaceae Hedyotis diffusa Hedyotis for the genus Hedyotis diffusa Hedyotis diffusa Willd. Dry the whole plant, the main producing provinces in the south of the Yangtze River in China. This product has a heat-clearing and detoxifying, promoting blood circulation Huayu, dampness such as the role of Tonglin, mainly for cancer, jaundice, hepatitis, enteritis, pneumonia and other diseases. Hedyotis diffusa contains anthraquinones, flavonoids, iridoid glycosides terpenoids, steroids, phenolic compounds [1 ~ 3]. About the chemical constituents of Hedyotis diffusa analysis and determination of the active ingredient against which the majority of oleanolic acid and ursolic acid. Modern pharmacological studies have shown that phenolic compounds have significant anti-oxidation, the role of scavenging free radicals, with anti-inflammatory, antibacterial, anti-cancer effects [4], consistent with the role of Hedyotis diffusa. This the first time, has been isolated from Hedyotis diffusa phenolic compounds, 3,4 - 2, parabens, and t