High Performance Liquid Chromatography Guanxin Danshen Dropping Pill Dan acid content of B.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography Guanxin Danshen Dropping Pill Dan acid content of B [Abstract] Objective To determine the Coronary Danshen Dropping Pill salvianolic acid B content. Methods HPLC method, ODS2-C18 column (250 mm * 4.6 mm, 5 μ m), mobile phase: (methanol - acetonitrile): (formic acid - water) = 37:63, where methanol: acetonitrile = 3:1, formic acid: water = 1:29; flow rate of 1.0 ml * min-1; detection wavelength was 286 nm. Results Sal B concentration in the range of 0.1 ~ 0.9 μ g good linear relationship (r = 0.999 5, n = 5). Recovery was 101.56%, RSD = 2.86% (n = 6). Conclusion The experimental extraction Sal B treatment method is simple, stable and reliable measurement results can be used as coronary heart Danshen Dropping Pill Sal B, quantitative analysis method. [Keywords:] Coronary Danshen Dropping Pill; Sal B; high-performance liquid chromatography Abstract: ObjectiveTo quantitatively determine salvianolic acid B in Guanxindanshen Drop pills. MethodsThe HPLC procedure was used in the determination, the chromatographic column was ODS2-C18 (250 mm * 4.6 mm, 5μ m), the mobile phase was (methanol-acetonitrile): ( formic acid-water) = 37:63, and methanol: acetonitrile = 3:1, formic acid: water = 1:29. The flow velocity was 1.0 ml * min-1, and the detection wavelength was 286 nm. ResultsIt showed that salvianolic acid B had a good linear relationship when its concentration was in the range of 0.1 ~ 0.9μ g (r = 0.999 5, n = 5); and the sample recovery of the procedure was 101.56% (RSD = 2.86%, n = 6 ). ConclusionThe method is simple and accurate. It can be used for extraction and determination of salvianolic B in Guanxindanshen Drop pills. Keywords:: Guanxindanshen Drop pills; Salvianolic acid B; HPLC Guanxin Danshen Pill from Salvia, 37, descending the composition of sesame oil, with blood stasis, qi analgesic effect. Qi stagnation blood stasis due to the Chest for the treatment of heartache. Sal B for the Dans