High Performance Liquid Chromatography God Corning Pill catalpol content.docVIP

 PAGE \* MERGEFORMAT 6 High Performance Liquid Chromatography God Corning Pill catalpol content [Abstract] Objective: To determine God Corning Pill catalpol content. Methods: The high-performance liquid chromatography with octadecyl silane bonded silica gel as a filler, the mobile phase of acetonitrile: 0.1% phosphoric acid (two ninety-eight), flow rate 1.0ml/min, detection wavelength 210nm. Results: The God Corning pills catalpol content in terms of Rehmannia 0.4mg / g, recoveries were 97.7% (n = 5). Conclusion: The high-performance liquid chromatography method can be used to evaluate the quality of God Corning pills. [Keywords:] high-performance liquid chromatography; God Corning pill; Catalpol God Corning pills from the ground and yellow, Suanzaoren, yams, etc. composed of 12 Chinese herbal medicines, mainly containing Catalpol, saponin, ferulic acid and so on. A Qingrejiedu, Ziyin nourishing, Pinggan sedative effect. In this paper, high-performance liquid chromatography God Corning Pill catalpol content, chromatographic peak separation is good, method is simple and accurate. To control the intrinsic quality of the drug provides a reliable method. An instrument and reagent 1.1 Instrument performance liquid chromatography (Shimadzu), SPD  10Avp, Zhejiang University N2000 chromatography data workstation. 1.2 reagent Catalpol reference substance (110808  200407) by the Chinese biological products available reagents were used for HPLC pure. 2 Methods and Results 2.1 Chromatographic conditions using octadecyl silane bonded silica gel as a filler; acetonitrile: 0.1% phosphoric acid (two ninety-eight) as mobile phase: column temperature 35 ℃ ; flow rate was 1.0ml/min; detection wavelength 210nm. 2.2 Preparation of reference substance solution Solution Preparation: Take Catalpol reference substance 6mg, precision, said determined set 10ml Liang Ping, add water, dissolve and dilute to the scale, shake. Precise quantity take 2ml, home 10ml Liang Ping, add