High Performance Liquid Chromatography Danshen extract three kinds of water-soluble components in.docVIP

 PAGE \* MERGEFORMAT 13 High Performance Liquid Chromatography Danshen extract three kinds of water-soluble components in 【Abstract】 Objective To establish a high-performance liquid chromatography (HPLC) determination of water-soluble Danshen extract components Danshensu, protocatechuic aldehyde and Dan acid B-content. Method of high-performance liquid chromatography gradient elution method. The analytical column was Hypersil ODS C18 column (250 mm × 4.6 mm, 5 μm); mobile phase of 1% acetic acid solution (A) and methanol (B) gradient elution; detection wavelength was 281 nm; flow rate of 1.0 ml * min-1; column temperature was 25 ℃. Results Danshensu, protocatechuic aldehyde and Sal B respectively in the linear range of 0.26 ~ 2.08 μg, 0.13 ~ 1.04 μg, 0.45 ~ 3.60 μg good linear relationship (r ≥ 0.999 6); recoveries were 97.76%, 99.56%, 99.68%, RSD lt;3.4%. Conclusion The method is simple, sensitive, reproducible, and can be used for quality control of Danshen extract. Keywords: Salvia extract; water-soluble components; high-performance liquid chromatography Abstract: ObjectiveTo develop an HPLC method for determination of 3 water-soluble components-danshensu, protocatechuic aldehyde and salvianolic acid B in the extract from Salvia miltiorrhiza Bge.Methods The analysis was carried on with a column of Hypersil ODS C18 (250 mm × 4.6 mm, 5 μm) by HPLC at 25 ℃, with gradient elution of 1% (v / v) acetic acid (A)-methanol (B) as the mobile phase. The flow rate was 1.0 ml * min-1 and the detection wavelength was 281 nm. ResultsThe linear ranges of danshensu, protocatechuic aldehyde and salvianolic acid B were 0.26 ~ 2.08 μg, 0.13 ~ 1.04 μg and 0.45 ~ 3.60 μg respectively, and all the compounds showed good linearity (r ≥ 0.999 6) in corresponding detected concentration range. The average recoveries were 97.76%, 99.56%, 99.68%, and RSD was less than 3.4%. ConclusionThe method is simple, sensitive, accurate, reproducible, and can be used to control