High Performance Liquid Chromatography Actinidia deliciosa roots in Guangxi middle of ursolic acid.docVIP

 PAGE \* MERGEFORMAT 15 High Performance Liquid Chromatography Actinidia deliciosa roots in Guangxi middle of ursolic acid Author: Leung Kit-Han-Shen Zhen Sheng-mao Ma Wen-fang, LIU Zhen-jie Zhang Ting [Abstract] Objective To determine the root of Actinidia deliciosa in Guangxi middle of ursolic acid. Method to examine indicators of ursolic acid content, using orthogonal extraction process optimization. Using high performance liquid chromatography column was Thermo Hypersil BDS C18 column (5 μ m, 4.6 mm * 250 mm); column temperature 20 ℃ ; mobile phase of acetonitrile -0.1% phosphoric acid solution (85:15); flow rate of 1.0 ml / min; detection wavelength was 210 nm. The results of the concentration of ursolic acid 0.012 5 ~ 0.150 0 mg / ml with the peak area showed good linear relationship between the samples, the average recovery rate of 99.35%, RSD of 1.52% (n = 6). Conclusion The method is accurate and reliable, reproducible and applicable to the root of Actinidia deliciosa quality control of medicines. [Keywords:] high-performance liquid chromatography in Guangxi middle Actinidia deliciosa root ursolic acid Abstract: ObjectiveTo determine the ursolic acid in the roots of Actinidia deliciosa in Guangxi province by HPLC. MethodsWith the content of ursolic acid as index, orthogonal experiments were used to optimize extraction technology. HPLC method was developed. The separation was performed on Hypersil BDS C18 column (5 μ m, 4.6 mm * 250 mm). The column temperature was 20 ℃ . The mobile phase consisted of acetonitrile-0.1% phosphoric acid (85:15). The flow rate was 1.0 ml / min. The detection wavelength was 210 nm.ResultsThe result showed that there was good linear relationship between the area and concentration of ursolic acid within the range of 0.012 5 ~ 0.150 0 mg / ml. The average recovery was 99.35% and RSD was 1.52% (n = 6). ConclusionThe method is convenient, sensitive and reliable. It can be used for quality control of Actinidia deliciosa. Keyw