High Performance Liquid Chromatography - Diode Array Detection Method Yuanhua Fingerprint.docVIP

 PAGE \* MERGEFORMAT 10 High Performance Liquid Chromatography - Diode Array Detection Method Yuanhua Fingerprint Author: Tian Yuan-Zi Li Rao Rao Lei Peilin Lan Huang Lan Si-Tong Liu Ying [Abstract] Objective To establish the HPLC fingerprint of herbal medicine Yuanhua analysis. Methods Diamond  C18 column (4.6 mm * 250 mm, 5 μ m), methanol  water gradient elution, the volume flow rate 1 ml / min, column temperature 35 ℃ , detection wavelength 332 nm, recording time 50 min. To genkwanin as a reference compound, were determined for 17 batches of samples and calculates the similarity. The results of the initial establishment of the fingerprint Yuanhua herbs, calibration a total of seven peaks, the origin of the similarity between the good herbs. Conclusion The method is reliable, simple and feasible, can improve the Yuanhua medicines quality control standards. [Keywords:] Yuanhua RP-HPLC fingerprint of herbal medicine similarity Abstract: ObjectiveTo establish the analytical method for the fingerprint of Flos Genkwa by HPLC  DAD. Methods HPLC condition: column Diamond  C18 (4.6 mm * 250 mm, 5 μ m), the mobile phase was mixtures of methanol and water in gradient elution mode , the flow was 1 ml / min, the column temperature was 35 ℃ , the detection wavelength was set at 332 nm, and record time was 50 min. Genkwanin was used as the reference compound and 17 batches of Flos Genkwa were determined. Results7 co  possessing peaks were selected as the common peaks, and the similarity of the fingerprint was close in different growing region. ConclusionThis method is simple, accurate and can provide scientific reference for further quality control of Daphne genkwa Sieb. et Zucc. Keywords:: Flos Genkwa; HPLC-DAD; Fingerprint; Similarity Daphne genkwa for Thymelaeaceae plants Yuanhua Daphne genkwa Sieb. Et Zucc.’s Dry bud, by the traditional water-diarrhea medicine, toxic, with a drip-by-drink, detoxification insecticidal efficacy of oral administration for