High Performance Liquid Chromatography rheumatoid bone capsule of Strychnine and brucine alkali content.docVIP

 PAGE \* MERGEFORMAT 8 High Performance Liquid Chromatography rheumatoid bone capsule of Strychnine and brucine alkali content [Abstract] Objective To establish the rheumatoid bone capsule of two active ingredients in strychnine and brucine method for the determination. Methods RP-HPLC, Hypersil BOD C18 (5 μ m, 250 mm * 4.6 mm); mobile phase: 0.3% triethylamine aqueous solution (phosphoric acid adjusted pH 2.8) - acetonitrile: (88:12). Flow rate 1 ml * min-1; column temperature was 35 ℃ ; detection wavelength of 254 nm. The results of strychnine and strychnine injection volume, respectively 0.010 8 ~ 0.345 6 μ g, and 0.009 2 ~ 0.292 8 μ g peak area within the range of a good linear relationship, recoveries were 100.4% and 97.78%. Conclusion The method is simple, rapid, accurate and suitable for rheumatoid bone capsule of the quality control. [Keywords:] high-performance liquid chromatography with rheumatoid bone capsule a disability-ning MA brucine Rheumatoid bone capsule is ephedrine, brucine, wind, etc. Achyranthes compound consisting of 16 Chinese herbal medicines preparation by rheumatism bone Tablet [1] derived by changing the capsule dosage forms, with Herd cold-dispelling, dehumidification analgesic effect. Strychnos the main ingredients of strychnine and strychnine, its toxic and has to make a variety of central nervous system stimulant effects, safety scope is narrow, often leads to excessive use of convulsions, convulsions, coma and other serious nervous system adverse reactions [2,3]. Determination of methods have been reported thin-layer chromatography, UV spectrophotometry, HPLC, etc. [4,5]. In order to more effectively control the content, improve product quality, the present study, levels of both high-performance liquid chromatography. A Reagents and Drugs Days of the US high-performance liquid chromatography; 1000 spectrum workstation. Strychnine reference substance (Lot 0705-200005) were purchased from China Pharmaceutical and Biologica