High Performance Liquid Chromatography Simultaneous Determination of matrine and oxymatrine Kushen film content.docVIP

 PAGE \* MERGEFORMAT 13 High Performance Liquid Chromatography Simultaneous Determination of matrine and oxymatrine Kushen film content Author: Jiang Yanling, ZHANG Shan, Hui-sub - Keywords: Kushen tablets;,, oxymatrine;,, matrine;,, high-performance liquid chromatography Abstract: Objective To establish the oxidation of matrine and matrine in matrine film method for the determination. Methods using high performance liquid chromatography, using Thermo column, acetonitrile - phosphate buffer solution (taking potassium dihydrogen phosphate 1.36g, plus 1 ml triethylamine, add water to 1000 ml, adjusted with phosphoric acid pH5.5) (3:97) as mobile phase, flow rate of 1.0 ml / min, detection wavelength was 220 nm. Results oxymatrine in 0.005 5 ~ 0.088 mg / ml and the peak area between the integral value showed a good linear relationship (r = 0.999 8), average recovery was 99.41%, RSD 1.28%; matrine in the 0.106 ~ 0.170 mg / ml and the peak area between the integral value showed a good linear relationship (r = 0.999 9), average recovery was 99.35%, RSD = 1.25%. CONCLUSION The method is sensitive, accurate, specific, reproducible, can be used as quality control of Sophora tablets. Keywords:: Sophora flavescens plate; oxymatrine; matrine; high-performance liquid chromatography Abstract: ObjectiveTo establish a method for simultaneous determination of the content of matrine and oxymatrine in Kushen Tablets. MethodsHPLC method was carried out through Thermo C18 column, using acetontrile-phosphate buffer solution (contained 1.36 g potassium dihydrogen phosphate and 1 ml triethylarmine, add water to 1000 ml, adjusted to pH 5.5 with phosphate acid) (3:97) as mobile phase. The flow rate was 1.0 ml / min with detective wavelength of 220 nm. ResultsThe calibration curve of oxymatrine content was linear within the range of 0.0055 ~ 0.088 mg / ml (r = 0.999 8), the average recovery of oxymatrine was 99.41%, RSD was 1.28%; the calibration curve of mat