High Performance Liquid Chromatography skin mimatsu cream triamcinolone acetonide acetate content of.docVIP

 PAGE \* MERGEFORMAT 7 High Performance Liquid Chromatography skin mimatsu cream triamcinolone acetonide acetate content of [Abstract] Objective To develop a skin cream in the US-song acetate method for the determination of triamcinolone acetonide. Method of high-performance liquid chromatography with octadecyl silane bonded silica gel as a filler; with methanol - water - ether (75:25:2) as mobile phase; detection wavelength was 240 nm; theoretical plate number by triamcinolone acetonide acetate pk calculation should not be less than 5000. An average recovery of 99.6% (RSD = 1.0%). Conclusion The method is simple, accurate, reproducible, and can be loose as a skin cream in the United States and triamcinolone acetonide acetate method for quality control. [Keywords:] triamcinolone acetonide cream skin US-song performance liquid chromatography Skin Cream for the US-song Second Hospital of Huangshi City, Hubei Province, home-made preparations, mainly by triamcinolone acetonide acetate, urea, camphor and other ingredients. Is mainly used for atopic dermatitis, skin keratosis, hand, foot and chapped and dry skin pruritus. None of the original standard assay methods, in order to better control the drug quality, this study established a high-performance liquid chromatography peptide mimatsu cream triamcinolone acetonide acetate content in new ways. Are reported below. An instrument and reagent SSIS PC3000 high-performance liquid chromatography; Mettler parts per million balance (AG135). Skin Meisong cream (homemade preparations hospital), lot number 070201,070202,070203; acetate, triamcinolone acetonide reference substance by the Chinese pharmaceutical and biological products to offer Lot :100125-0103; HPLC pure methanol, ether for the analysis of pure. 2 Methods and Results 2.1 Chromatographic conditions Column: Kromasil C18 column (4.6 mm * 250 mm, 5 μ m); mobile phase: methanol - water - ether (75:25:2) as mobile phase; detection wavelength: 240 nm; flow rat