HPLC Danshen drip fluid content of Sal B.docVIP

 PAGE \* MERGEFORMAT 5 HPLC Danshen drip fluid content of Sal B Author: Tax Pi Xian Zhuang Yuan Chun Bing Zhu Ye [Abstract] Objective To establish a high-performance liquid chromatography (HPLC) determination of Salvia infusion fluid salvianolic acid B content approach. Method column Dikma Kromasil C18 (5 μ m, 100A 250 mm * 4.6 mm); mobile phase of methanol - acetonitrile - formic acid - water (30:10:1:59); flow of 0.8 ml * min-1; detection wavelength 286 nm; column temperature 30 ℃ ; injection volume 20 μ l. Dan B, plus the results of acid extracted sample average recovery was 99.03%, RSD 1.02%, RSD of repeatability experiment 1.8l%. Conclusion The method is simple and reliable, the results of stability, good reproducibility, the method can be used to determine the Salvia infusion fluid salvianolic acid B content. [Keywords:] Sal B high performance liquid chromatography determination of Salvia infusion fluid Salvia infusion of fluid contained in the national income standard compilation of proprietary Chinese medicine in mind and volumes, from the Danshen water-soluble compounds extracted from the total Sal obtained. Chief Dan acids with anti-hepatic fibrosis, anti-atherosclerosis and improve the role of memory dysfunction, etc. [1], by Danshensu, Sal A, B, E, protocatechuic aldehyde and various aromatic phenolic acids compounds formed. Salvia drip liquid standards right Danshensu sodium, protocatechuic aldehyde conducted a high-performance liquid chromatography assay, of which high levels of active and strong Sal B did not carry out assay. We refer to the literature [2,3], measured by high performance liquid chromatographic column drip fluid Dan Danshen phenolic acids content of B to obtain satisfactory results. 1 Equipment 1.1 Instrument and reagents Dianne high-performance liquid chromatography (P680A quaternary pump, PDA-100 diode array detector, TCC-100 oven, Chromeleon chromatography workstation). Sal B reference substance (China provided medicines