HPLC Determination of Vitamin E Tablets.docVIP

 PAGE \* MERGEFORMAT 8 HPLC Determination of Vitamin E Tablets Author: on behalf of Yang Wan-Qing Miao Zhao Jing Zhu Jun [Abstract] Objective To establish the determination of vitamin E (VitE) content in high-performance liquid chromatography. Methods Shimadzu C18 column (4.6 mm * 250 mm, 5 μ m), with methanol as the mobile phase; detection wavelength was 284 nm, flow rate 1.5 ml * min-1. The results of vitamin E in the 0.125 ~ 5.0 mg * ml-1 within the peak area with the linear relationship was good (r = 0.999 9), high, medium and low concentrations of three kinds of average recovery was 101.4% ~ 102.7% , RSD 0.46% ~ 0.71%, the lowest detection limit of 0.4μ g * ml-1. Conclusion This method is accurate, simple, rapid and applicable to determination of vitamin E tablets. [Keywords:] of vitamin E; high-performance liquid chromatography; determination Abstract: Objective To establish a RP-HPLC for determination of Vitamin E. Method Vitamin E was determinated on HPLC instrument fixed a Shimadzu C18 column (4.6mm * 250mm, 5μ m) with UV detection at 284nm, and the mobile phase was MeOH, the flow rate was 1.5ml * min-1.Results The calibration curve was linear (r = 0.9999) within the range of 0.125 ~ 5.0 mg * ml-1 for Vitamin E, the average recovery rates were from 101.4% to 102.7% for three different levels of the amount of Vitamin E, RSD was from 0.46% to 0.71%, and LOD was 0.4μ g * mL-1.Conclusion This method was simple, quick, accurate and effective for determination of Vitamin E. It was suitable for the quality control for tabella Vitamin E. Keywords:: Vitamin E; HPLC; determination Vitamin E also known as tocopherol, is a fat-soluble substance in the human body mainly involved in reproductive function, muscle metabolism and other processes, a powerful antioxidant, one of the body’s essential trace elements. At present the main analytical methods include VitE chemiluminescence inhibition assay [1], UV method [2], GC method [3], fluorescence [4] and H