HPLC method for simultaneous determination of puerarin and cold cold tablets hesperidin.docVIP

 PAGE \* MERGEFORMAT 8 HPLC method for simultaneous determination of puerarin and cold cold tablets hesperidin [Abstract] Objective To establish the determination of cold cold tablets. Methods using HPLC, column for the Diamond C18 (4.6 mm 250 mm, 5 m, mobile phase of methanol water (25:75, detection wavelength: Puerarin 250 nm, hesperidin 283 nm. the results of puerarin injection volume was 0.156 ~ 3.152 g range of hesperidin in the sample volume was 0.100 ~ 2.006 g range, with peak area was a good linear relationship between the average recoveries: puerarin was 99.0%, RSD = 0.97%, hesperidin was 98.4%, RSD = 1.27% (n = 9. Conclusion The method is simple, accurate and reliable for the quality of cold flu tablets control. [Keywords:] cold flu tablets puerarin HPLC hesperidin Cold cold tablets by ephedra, arrowroot, basil leaves, wind, dried tangerine peel, Angelica other eleven herbs processed by the extraction of the tablet, with a solution table sweating, the role of wind-dispersing and cold and clinical for a cold and fever , headache, cough, nasal congestion, runny nose. In this paper, HPLC method for simultaneous determination of puerarin in this product contains, the content of hesperidin as a cold cold tablets quality control targets. 1 equipment, reagents and sample Waters2695 HPLC, Waters2487 UV detector (Waters Corporation USA; Milli PORE pure water (in the United States close his company. Puerarin reference substance, reference substance hesperidin (China Pharmaceutical and Biological Products, used for determination , methanol HPLC grade water for high water, other reagents were of analytical grade, cold cold tablets sample (size: 0.6 g per tablet weight and lack of arrowroot, dried tangerine peel the negative control samples from Guangxi Yue Hui Pharmaceutical Co., Ltd. to provide. 2 Methods and Results 2.1 Diamond C18 column chromatography conditions (5 m, 4.6 mm 250 mm), mobile phase of methanol water (25:75, detection wa