Human prostate cancer PC3 cell subsets Isolation and behavioral analysis.docVIP

 PAGE \* MERGEFORMAT 11 Human prostate cancer PC3 cell subsets Isolation and behavioral analysis Study: School Song Shanshan Li Ronggui this Haiying Wang Yang Su Li Yulin Abstract Objective To study the human prostate cancer PC3 tumor cell lines with stem cell characteristics of the existence of subpopulations, and their morphology and growth characteristics of clones analyzed, while color intensity of dye Rho123 it can be used to distinguish different subsets of tumor cells . Methods Rho123 staining and flow cytometry; Rho123 after stained with DAPI fluorescence microscope and cell morphology analysis, clonal growth characteristics. Results Rho123 PC3 cells in the presence of two significant differences in color cell subsets, their morphology, proliferation and clonal growth patterns were significantly different. Conclusion PC3 human prostate cancer cell lines with stem cell properties exist subsets of features can be dyed Rho123 refusal to distinguish cancer stem cells and other tumor cells. Keywords: prostate cancer; rhodamine 123; cancer stem cells Abstract Objective To identify the characteristic of cancer stem like cells in prostatic carcinoma PC3 cell line, and analyze the morphous and clone growth, investigate the method of discriminating the different tumor cell subpopulation by Rhodamin123 dye. Methods The PC3 cells stained with Rhodamin123 dye were analyzed by flow cytometry; the cells morphous and clone growth stained with Rhodamin123 and DAPI were observed by fluorescent microscope. Results Two different tumor cell subpopulation existed in PC3 cell line, and the morphous, reproductive activity and colon growth were significantly different in the two cell subpopulation. Conclusions Cancer stem like cell subpopulation exists in prostatic carcinoma PC3 cell line, the character of some cells excluding Rhodamin123 dye can be used to discriminate tumor stem cells and other tumor cells. Keywords: Prostatic carcinoma; Rhodamin123; Cancer