ICAM1 in experimental autoimmune encephalomyelitis in the pathogenesis of the role of.docVIP

 PAGE \* MERGEFORMAT 11 ICAM1 in experimental autoimmune encephalomyelitis in the pathogenesis of the role of Of: Ya-Pu WANG LIU Hui-min Wang Xiuqing Huang Qingjun [Abstract] Objective To determine the experimental autoimmune encephalomyelitis (EAE) rat blood-brain barrier endothelial cells in the intercellular adhesion molecule 1 (ICAM 1) expression, and to analyze the expression of EAE pathogenesis. Methods EAE group and the control group, brain tissue, paraffin sections, immunohistochemistry, image scanning system of observation, the gray-scale image analysis software. Results EAE rat brain vascular endothelial cells ICAM 1 expression was significantly darker than the control group. experimental group is 141.47 + -6.88 gray, while the control group was 166.24 + -7.24, the difference was significant (P lt;0.01). Conclusion EAE rats in brain vascular endothelial cells increased the expression of ICAM 1, suggesting ICAM 1 is to be associated with EAE important adhesion molecules. [Keywords:] immunohistochemistry, ICAM 1, blood-brain barrier, experimental autoimmune encephalomyelitis [Abstract] Objective To investigate the relationship between the expression of ICAM 1 in the blood brain barrier and the experimental autoimmune encephalomyelitis (EAE). Methods Brain of EAE as well as control animals were taken and embedded in paraffin.And the sections were stained by immunohistochemical method. Then the sections were observed by Nikon image scanning system and measured the gray value by IPP image analysis software. Results The expression of ICAM 1 in the epithelial cell of endangium of EAE rat’s brain was higher than that in control. Conclusion The increase of the expression of ICAM 1 in the epithelial cell of endangium of EAE rat’s brain indicated that ICAM 1 was a necessary adhesion molecule in the developmental process of EAE. [Keywords:] Immunohistochemical method, Intercellular adhesion molecular 1, Blood brain barrier, Experimental autoimmune