IL11 protection after neutron irradiation damage in intestinal epithelial Jak - STAT signal transduction mechanism.docVIP

 PAGE \* MERGEFORMAT 16 IL11 protection after neutron irradiation damage in intestinal epithelial Jak / STAT signal transduction mechanism Of: Wang Ruijuan, Peng Ruiyun, high Asian soldiers, often citizens, Xu Xinping, Fukai Fei, Luo Qingliang [Abstract] Objective: To investigate the IL 11 on neutron irradiated intestinal intraepithelial Jak / STAT pathway, and to observe its effect on the expression of Bax and Bcl 2. Methods: BALB / c mice and IEC 6 cells were treated with 4 Gy in processing sub-irradiation and IL 11, respectively, as intestinal epithelial injury in vitro and in vivo model, using HE staining, Western blot, EMSA, immunohistochemistry and image analysis of intestinal pathological changes were detected, Jak1 and STAT3 activity and the expression of Bax and Bcl 2 . Results: (1) neutron irradiation, a very serious intestinal injury in mice, and no significant regeneration; IL 11 treatment group and survival of crypt epithelial cells in crypt larger number. (2) neutron irradiation, IEC 6 cells reduced activity of Jak1 and STAT3, IL 11 treatment increased both activities. (3) neutron irradiation, increased intestinal expression of Bax, Bcl 2 expression did not change significantly; application of IL 11 to reduce Bax expression, increased Bcl 2 expression. Conclusion: The neutron radiation, IL 11 can activate the Jak / STAT pathway, and reduced Bax expression, increased Bcl 2 expression in intestinal epithelium play a protective role. [Keywords:] radiation; intestinal epithelium; IL 11; Jak / STAT; apoptosis [Abstract] AIM: To explore the effect of IL 11 on the activation of Jak / STAT pathway and the expressions of Bax and Bcl 2 in the intestinal epithelial cells exposed to neutron radiation. METHODS: The BALB / c mice and IEC 6, irradiated by 4 Gy neutron with or without IL 11 treatment, served as in vivo and in vitro model seperately. The changes of the intestines, activity of Jak1 and STAT3 and expressions of Bax and Bcl 2 were observe