Immunomagnetic Separation and purification of Schwann cells in vitro.docVIP

 PAGE \* MERGEFORMAT 19 Immunomagnetic Separation and purification of Schwann cells in vitro Author: Huang Jing Xiao Wei Qiang Ming Luozhuo Abstract [Objective] To investigate the use of immunomagnetic bead isolation and culture of rat sciatic nerve from the SD to obtain a large number of high-purity method of Schwann cells. [Methods] use 4 ~ 7 d SD rats were obtained under sterile conditions, bilateral sciatic nerve, anatomical dissection microscope to remove epineurium, access to nerve bundles, Shredded the nerve bundle to the size of 1 mm3. 2 dual-enzyme digestion digestion, fetal bovine serum suspension of digestion, centrifugation and joined the DF12 culture medium. 7 d after the application of immunomagnetic bead method, cultured cells were purified, cultured 2 d after inoculation passage. Develop during the course of cells were observed, counting and viability determination; MTT cell growth curve mapping method, using immunocytochemical staining cells were identified and calculated cell purity. [Results] shall be isolated and cultured cells derived from Schwann cells; using immunomagnetic bead method can be derived from Schwann cells cultured and purified. Purified Schwann cell viability of 96% ± 0.5%, purity 98% ± 1.1%, can be cultured 2 d after passage. [Conclusion] immunomagnetic bead method can be used for the purification of Schwann cells cultured Schwann cells derived from high purity and vitality of strong and can meet the need for artificial neural tissue engineering. Keywords: Schwann cell; immunomagnetic beads; sciatic nerve Abstract: [Objective] To introduce a method to obtain Schwann cells from newly born SD rats massively and purely by immunomagnetic heads method. [Method] SD rats that had been born within 5 to 7 days were used. Their bilateral sciatic nerves were dissected under sterile condition. Under 16 × microscope the nerve fascicles and the epineurium were carefully extracted in oder to get the nerve tract