Identification of species-specific DNA probes herbal method of screening.docVIP

 PAGE \* MERGEFORMAT 23 Identification of species-specific DNA probes herbal method of screening [Abstract] gene chip technology to Chinese herbal medicine efficient, parallel, provides the possibility for rapid identification. The Screening of Chinese herbal medicine species-specific probes are herbs gene chip identification technology developed by Key. In combination with today’s medicine identification chips and other species identification microarray summary of progress of several Chinese herbal medicine-specific probes suitable for screening methods. [Keywords:] gene chip; herbal identification; species-specific; DNA probe Abstract: DNA chip technology makes it feasible to identify Chinese herbal medicine rapidly, efficiently and in parallel. The screening of species-specific DNA probes from medicinal plants is the key step in developing DNA chip for the authentication of Chinese herbal medicines. Combined with current DNA chip researches on identification of herbal plants or other species, some methods available for the screening of medicinal plants’ species-specific DNA probes were summarized in this paper. Keywords:: DNA chip; Herbal medicine; Species-specific DNA Probe In traditional Chinese medicine characteristic gene identification and gene sequencing, based on the establishment of gene diagnosis and gene chips as the carrier of molecular identification techniques and methods of Chinese herbal medicine is the future of Chinese medicine biotechnology research efforts in the direction of one of [1]. Identification of the gene chip technology, traditional Chinese medicine refers to the in-situ synthesis, or micro-printing methods, the kinds of traditional Chinese medicine-specific DNA probes fixed on glass slides, nylon membrane or other support surface, forming two-dimensional array of DNA probes and then with the fluorescent marker for inspection or DIG genomic DNA samples of traditional Chinese medicine hybridization signals by detecting the hy