Identification of RAPD types in Gynostemma explore the conditions for application.docVIP

 PAGE \* MERGEFORMAT 12 Identification of RAPD types in Gynostemma explore the conditions for application Author: Arroyo, Li Xiong Ying, WU Yao-sheng, JIANG Jun-Fu, Zhao Ruiqiang, Zeng-Qi Yan [Abstract] Objective To identify random amplified polymorphism and its Analogues Gynostemma Cayratia japonica, to explore the conditions for RAPD. Methods to improve CTAB extraction Aescin Jiaogulan, Gynostemma leaf 5, Cayratia japonica, as well as the tobacco genomic DNA, to explore the impact RAPD conditions. The results obtained using the improved CTAB method of DNA extraction better quality, can be used RAPD amplification, and explored the optimal conditions for RAPD amplification. Conclusion improved CTAB method is suitable for these four kinds of plant DNA extraction, amplification conditions for RAPD groping obtained can be used to identify and Its Analogues Gynostemma types of goods Cayratia japonica difference. [Keywords:] Jiaogulan genomic DNA randomly amplified polymorphic DNA Identification Abstract: ObjectiveTo explore the conditions of the differentiation of Gynostemma pentaphyllum from the mixed plant, Cayratia japonica with RAPD technology. MethodsThe improved CTAB method was used to extract the genome DNA from Jiao Gu Lan (Gynostemma pentaphyllum) Seven Leaves Jiao Gu Lan, Cayratia japonica and Tobacco, respectively. The optimum conditions of RAPD were researched. ResultsWith the improved CTAB method, the extracted DNA presented better quality and could be used to progress RAPD. The optimum conditions for RAPD have been successfully explored. Conclusion The improved CTAB method is suitable to extract genome DNA from the four medicinal plants, respectively, and the conditions explored with RAPD technology can be used to identify the variety of Gynostemma Pentaphyllum and distinguish it from the mixed plant, Cayratia japonica. Keywords:: Gynostemma pentaphyllum; Genome DNA; RAPD; Identification Jiaogulan Gynostemma pentaphyllum, (Thunb) Makino Departme