Injection of recombinant hirudin product quality dual-function study of.docVIP

 PAGE \* MERGEFORMAT 23 Injection of recombinant hirudin product quality dual-function study of Zhang Yanling, Mo Wei-Δ, Long-Sheng Wang, Yang Ying, Hou-Yan Song Abstract constructed in our laboratory secreted high-efficiency dual-function of recombinant hirudin (RGD-Hirudin) engineering yeast by fermentation, purification, recombinant hirudin pure dual-function Its stock solution by the quality and analysis, in line with national standards of intravenous drug use, can be used to prepare intravenous injection. Keywords: dual-injection of recombinant hirudin; Quality Research 【Abstract】 The expression plasmid, HD-pPIC9K, was contructed by inserting cDNA of RGD-hirudfin (RGD-Hirudin) in yeast expression vector pPIC9K. This clone was fermented for 3 days, and the purified RGD-Hirudin was gained after purification. Quality analyses were tested. The RGD-Hirudin was measured up to standard of injection. Natural hirudin is a specific inhibitor of thrombin, from the medical leech extract derived from the salivary glands may be the formation of thrombin molar ratio of 1:1 combination of non-reversible covalent complex of thrombin has a high affinity, so that thrombin cleavage of fibrinogen to lose the ability to suppress the formation of fibrin, therefore, a low concentration of hirudin can effectively inhibit blood coagulation [1]. We retain the natural biological function of the original hirudin based on the integration of the RGD sequence encoding the natural hirudin cDNA gene [2 ~ 5]. Constructed RGD-containing coding sequence of hirudin cDNA clone HD-pPIC9K, expression plasmid was transformed into Pichia pastoris, the highly expressed strain obtained by screening and the establishment of engineering strain to achieve an efficient, Secretory expression (another paper). This article focuses on re-reported dual-function Solutions for Quality of hirudin. 1 Materials and methods 1.1