IQ200 automated urine microscopy analyzer and manual determination of red blood cells in urine compared.docVIP

 PAGE \* MERGEFORMAT 6 IQ200 automated urine microscopy analyzer and manual determination of red blood cells in urine compared [Keywords:] IQ200 automated urine analyzer; microscopic; red blood cells IQ200 automated urine analyzer to provide urine for clinical application of analysis of the data, is fast, simple, high degree of automation, large quantities of samples for testing and other advantages, it is widely used in clinical laboratory. In order to determine the IQ200 automatic urine analyzer detected in urine red blood cells the accuracy of the determination of the microscopic method with which to compare the results of a preliminary analysis as follows. 1 Materials and methods 1.1 The source of specimen pick up my morning urine of 100 hospitalized patients were, 2h the test is completed. Selected 20 patients with microscopic positive samples of calcium oxalate crystals. U.S. IRIS IQ200 1.2 equipment automatic urine analyzer and its reagents, FAST READ 10R sediment counting board, microscope, centrifuge, test tubes urinary sediment. 1.3 Detection 1.3.1 Urine specimens IQ200 automated urine analyzer according to the instructions of the 100 urine specimens for testing, and follow the lt;lt;Standardization of urine sediment examination “Recommendationsgt; [1], the method used to do parallel microscopic determined. take 10ml of urine in the centrifuge tube, 1 500 r / min centrifugation 5min, take 0.2 ml by adding FAST READ 10R Urine sediment examination board count, count 10 large squares (volume 1 l) in the number of red blood cells. microscopic method to the results as “gold standard”, and IQ200 automated urine analyzer the results were compared and analyzed. According to lt;lt;National Clinical Laboratory Proceduresgt;gt; results in the determination of urinary sediment in order to erythrocytesgt; 12 / l for the positive standard. 1.3.2 Interference test (1) 20 patients with microscopic positive samples of calcium oxalate with IQ200