Irbesartan inhibited high glucose-induced lipid oxidation effect and human umbilical vein endothelial cell injury.docVIP

 PAGE \* MERGEFORMAT 17 Irbesartan inhibited high glucose-induced lipid oxidation effect and human umbilical vein endothelial cell injury Of: Lu Min, Lu group, Ren Yan, Tian Gang [Abstract] Objective To study the effects of irbesartan on the high glucose-induced lipid oxidation effects and human umbilical vein endothelial cells (HUVEC) injury. Methods HUVEC grew well in the experiment. The cells were divided into 4 groups: normal glucose control (glucose final concentration of 5.5mmol / L), high glucose (glucose final concentration of 33.3mmol / L), irbesartan group and anti-oxidant N acetyl cysteine (N acetyl cysteine, NAC ) group. intervention group first with irbesartan (10-5mol / L) and NAC (10mmol / L) were pre-action 1h, and then add 33.3mmol / L glucose were incubated 24,48,72 h. TBARS method were used and the supernatant were detected spectrophotometer malondialdehyde (MDA) content and superoxide dismutase (SOD) activity. Results Compared with normal glucose, high glucose group was significantly higher MDA (P lt;0.01 ), SOD activity decreased significantly (P lt;0.01); 24h, high glucose levels have reached a higher level of MDA (P lt;0.01), and the role of glucose with time (48,72 h) MDA contents increased, but 24h high glucose, the difference was not significant (Pgt; 0.05). with the high glucose group compared with irbesartan group and NAC group were significantly decreased MDA content (P lt;0.05), SOD activity was significantly increased ( P lt;0.05). with the high glucose group compared with irbesartan group and NAC group at each time point cells were significantly lower (P lt;0.05), high glucose on HUVEC cells significantly reduced the damage morphology. Conclusion E Beisha Tanzania part of the role of oxidative stress by inhibiting the reduction of high concentrations of glucose induced lipid oxidation effect, protecting HUVEC. [Keywords:] Irbesartan; glucose; low-density lipoprotein; oxidative stress; atherosclerosis ABSTRACT: Objecti