Intracellular calcium in human gastric cancer cell hypoxia-inducible factor1 expression and transcriptional activation of the role of the.docVIP

 PAGE \* MERGEFORMAT 2 Intracellular calcium in human gastric cancer cell hypoxia-inducible factor1 expression and transcriptional activation of the role of the Author: Lan-mei, SHI Yong-Quan, HAN Yi, Ning Xuan, Dai-Ming Fan [Abstract] Objective To observe the changes in intracellular calcium regulatory subunit of oxygen HIF  1α expression and HIF  1 transcriptional activation effects. Methods normoxia, the adoption of the permeability of intracellular calcium chelator BAPTA  AM or the calcium ionophore Ionomycin reduced or elevated intracellular calcium, detected by Western  blot on SGC7901 cells, HIF  1α protein expression, Then using indirect immunofluorescence assay, double-luciferase reporter assay system and changes in cell ELISA of calcium on HIF  1α translocation, HIF  1 transcriptional activity and its target genes vascular endothelial growth factor (Vascular endothelial growth factor, VEGF) secretion impact. Results BAPTA  AM to reduce intracellular calcium, can induce the expression of HIF  1α to promote HIF  1α translocation and enhance the transcriptional activity of HIF  1 increase HIF  1 regulate gene VEGF secretion. Ionomycin increased with intracellular calcium, although there are slight to promote HIF  1α stability and translocation role, but HIF  1 transcriptional activity and no significant effect on the secretion of VEGF. Conclusion chelation of intracellular calcium in gastric cancer cells can be induced by HIF  1α expression and HIF  1 transcriptional activation, suggesting that changes in intracellular calcium in gastric cancer cells may be hypoxia signal transduction play an important role. [Keywords:] gastric cancer cells; intracellular calcium; hypoxia-inducible factor  1; protein expression; transcriptional activity Department of Internal Medicine, the Affiliated Hospital of Medical College of Shaoxing University, Shaoxing 312000, China; Institute of Digestive Disease