Irisquinone reversal of the effect of COC1-DDP.docVIP

 PAGE \* MERGEFORMAT 14 Irisquinone reversal of the effect of COC1/DDP OF: Ming-Hua Liu Xiao Shunhan Xiewei Rong Rong Cao Yang Ren Meiping [Abstract] Objective: To establish a cisplatin-resistant ovarian cancer cell line COC1/DDP, of irisquinone reversal of COC1/DDP. METHODS: The concentration of progressively DDP in vitro was induced by intermittent ovarian cancer cell line induced COC1, established cisplatin-resistant ovarian cancer cell line COC1/DDP; to CCK 8 kit (Cell Counting Kit 8) detected COC1/DDP irisquinone on cell proliferation; to GSH and GSSG kit of intracellular GSH level; to HPLC the content of intracellular cisplatin. Results: During the 5 months to establish COC1/DDP, stability of cisplatin resistance, resistance index 9.44, and carboplatin, vincristine and adriamycin have different levels of cross-resistance; COC1/DDP irisquinone on the reversal of cisplatin resistance with the role reversal fold to 3.44-fold; and COC1 comparison, COC1/DDP increased intracellular levels of GSH, cisplatin decreased However, after intervention by irisquinone, COC1/DDP the level of intracellular GSH decreased cisplatin were increased (P lt;0.01). Conclusion: There COC1/DDP irisquinone reverse effect on its mechanism may be intervention COC1/DDP cell GSH / GST π detoxification system, increase of intracellular cisplatin content. [Keywords:] irisquinone; COC1/DDP; cisplatin; drug resistance Ovarian cancer is cancer occurring in the ovary, accounting for about 15% of gynecological malignancies, with the onset occult, early difficult to find, easy to transfer and poor prognosis features, is the highest mortality of gynecological tumors. Advanced ovarian cancer with chemotherapy resistance and poor prognosis, characterized, and its five-year survival rate of only 25% to 30%. platinum drugs is currently the most widely used clinical anti-cancer drugs, one of its representative drug cisplatin (cisplatin, DDP) its broad-spectrum, highly effective anti-cancer