Isopropylidene mechanism of anti-inflammatory effects of shikimic acid.docVIP

 PAGE \* MERGEFORMAT 11 Isopropylidene mechanism of anti-inflammatory effects of shikimic acid Of: Chen Xiaojun Gu Ligang Shi Taiping Sun Jianning [Abstract]: Objective isopropylidene shikimic acid (ISA) on human cervical carcinoma Hela cell transcription factors STAT1, STAT3, NF-κB expression in the role, and to explore its anti-inflammatory mechanism. Methods MTT method in vitro testing of different ISA concentration on growth inhibition of Hela cells, dual luciferase reporter system, with different concentrations of drugs on the transcription factor STAT1, STAT3, NF-κB expression activity. the result of human cervical cancer Hela cells ISA growth was not inhibited , ISA treated NF-κB-luc luciferase activity decreased, while the GAS-luc, STAT3-luc luciferase activity was not changed. Conclusion ISA may be reduced by the transcription factor NF-κB activity, inhibit the expression of inflammatory cytokines, Inhibition of the inflammatory response play. [Keywords:] shikimic acid isopropylidene Hela cells NF-κB anti-inflammatory Abstract: Objective To study the effect of 3,4-oxo-isopropylidene-shikimic acid (ISA) on transcription factor STAT1, STAT3 and NF-κB in human cervix cancer Hela cell. Methods The cell proliferation was assessed by MTT assay. The luciferase activity of transcription factor STAT1, STAT3 and NF-κB was determined by the Dual-Luciferase Reporter Assay System. Results ISA can not inhibited the growth of Hela cell. The luciferase activity of transcription factor NF-κB in Hela cells treated with ISA was inhibited , while the luciferase activities of transcription factor STAT1 and STAT3 were not inhibited. Conclusion ISA can inhibit inflammation, which may be related with suppression of NF-κB transcriptional activity. Keywords: :3,4-oxo-isopropylidene-shikimic acid, NF-κB, anti-inflammation, Hela cell Isopropylidene shikimic acid (3,4-oxo-isopropylidene-shikimic acid, ISA) is extracted from star anise Magnoliaceae active ingredie