Jiawei Sini Decoction on liver fibrosis in rat liver tissue transforming growth factor 1 connective tissue growth factor mRNA of the study.docVIP

 PAGE \* MERGEFORMAT 9 Jiawei Sini Decoction on liver fibrosis in rat liver tissue transforming growth factor 1 connective tissue growth factor mRNA of the study Of: Zhengxu Rui, Ye Jian-hong, Zhou Xinying, YANG Yu, Shou-only [Abstract] Objective To observe the liver Jiaweisinisan transforming growth factor 1 (TGF 1, connective tissue growth factor (CTGF mRNA, and to explore the mechanism of liver fibrosis. Methods Preparation of intraperitoneal injection of dimethylnitrosamine rat liver fibrosis model, intragastric administration of MSP, the use of real-time quantitative PCR technique to detect the liver tissue expression of TGF 1 and CTGF mRNA levels. Results MSP can significantly reduce liver fibrosis in rats liver tissue levels of TGF 1 and CTGF mRNA, and the pathological model group were significantly different (P lt;0.05. Conclusion MSP can reduce the liver expression of TGF 1 and CTGF mRNA, which play a role in its anti-fibrosis. [Keywords:] MSP liver fibrosis induced by transforming growth factor connective tissue growth factor MSP is an effective treatment of chronic viral hepatitis experience side, past the clinical observation and animal experimental studies have confirmed that the party has a good role in the prevention and treatment of liver fibrosis [1 ~ 3]. This experiment dimethylnitrosamine ( intraperitoneal injection of DMN induced liver fibrosis rat model, real time quantitative PCR in liver tissue detected by transforming growth factor 1 (TGF 1, connective tissue growth factor (CTGFmRAN expression of MSP hepatic fibrosis mechanism. 1 Materials 1.1 Animals SD 60 rats, male, clean, body weight (180 + -20) g, by the Experimental Animal Center of Fourth Military Medical University purchased. 1.2 Drugs and reagents MSP decoction of the Bupleurum, Citrus aurantium, white peony root, licorice, astragalus, red sage root, peach kernel and other drug composition, since the purchase of Shaanxi Traditional Chinese Medicine Affiliate