Jinzhou City the project nearly six outpatient TB staining of acid-fast bacilli.docVIP

 PAGE \* MERGEFORMAT 5 Jinzhou City the project nearly six outpatient TB staining of acid-fast bacilli [Keywords:] Jinzhou City, nearly six years of tuberculosis acid-fast bacilli staining Mycobacterium tuberculosis belongs to genus, Guo Huo 1882 sputum samples from patients with pulmonary tuberculosis were found. Is a major pathogen of human tuberculosis. Mycobacterial growth was branching, higher nutritional requirements than ordinary bacteria, is a slow growth Gram-positive bacteria. by phenol red dye staining complex, resistant to hydrochloric acid alcohol decolorization, it is called acid-fast bacteria such bacteria. acid-fast bacilli bacteria main cause of tuberculosis, of which TB is most common, so strengthening the suspected TB investigation in patients with acid-fast bacilli, for the identification of TB patients and to determine whether infectious, treatment is especially important significance. At present, the world promoting the use of direct supervision of the DOTS strategy, and the bacteriology laboratory diagnosis strategy for the direct supervision of a key short-course chemotherapy on the success of TB control programs play an important role. 1 Subjects and methods 1.1 Object Jinzhou City TB program in 2003-2008 were 7244 out-patient, aged 16 to 92 years, male 656, female 6588 people. 1.2 Methods 1.2.1 luxuriant - Nigeria’s anti-acid stain. For different sputum samples were stained by direct thick. Reagents used: Chu Hai Beisuo by the luxuriant Biotechnology Limited - Nigeria’s (Ziehl-Neelesen tech-nique) staining reagents. Phenol red dye complex: the same single-staining, but without using distilled water. Bleaching agent: alcohol hydrochloride. Complex dye: (Lu methylene blue solution) with the single staining. 1.2.2 staining (Celosia argentea - Nissl staining). Each slide can only use 1. The requirements of slides: one slide only a sputum smear may not be used again after cleaning smears, glass slides