Liquid chromatography mass spectrometry determination of sugar-free foods fructose glucose sucrose maltose and lactose.docVIP

 PAGE \* MERGEFORMAT 12 Liquid chromatography mass spectrometry determination of sugar-free foods fructose glucose sucrose maltose and lactose Of: Wang Hao Liang Yan Lei Shihai MING YANG Mei Guoqi Longbao [Abstract] established a sugar-free food in the glucose, fructose, sucrose, maltose, lactose, high performance liquid chromatography tandem quadrupole mass spectrometry method. The method to extract samples of water to Waters Carbohydrate Analysis column (300 mm × 3.9 mm, 10 μm mobile phase of water acetonitrile (15:85, V / V, negative ion electrospray ionization MRM mode detection. The detection limit was 0.1 g / kg, the linear range of 2.5 ~ 125.0 mg / L, the recovery rate was 86.2% to 97.7% with relative standard deviations of 3.1% to 8.7%. [Keywords:] LC-MS, sugar-free food; glucose; fructose; sucrose; maltose; lactose Abstract A LC MS MS method was developed for the determination of glucose, fructose, sucrose, maltose and lactose in sugar free products. The mass spectrometer was operated in the negative mode using MRM. In this method, samples were extracted with water and chromatographic separation was carried out using a Waters Carbohydrate Analysis column (300 mm × 3.9 mm, 10 μm) with a mobile phase of acetonitrile water (85:15, V / V). The limit of detection is 0.1 g / kg and the linear range was obtained from 2.5 to 125.0 mg / L. Overall recoveries were between 86.2% and 97.7%, and RSD value were between 3.1% and 8.7%. Keywords Liquid chromatography tandem mass spectrometry; Sugar free products; Glucose; Fructose; Sucrose; Lactose 1 Introduction 70 years of the 20th century, sugar-free food come out. The first sugar-free food is removed or added sugar in food salt, with the continual emergence of new sweeteners, sugar-free foods have developed rapidly from the initial dozen varieties development to the million. of mandated standards GB 13432 2004 lt;lt;Pre-packaged food labels for special dietary uses Code “gt; provides that: only