Liposome-mediated VEGFC antisense oligonucleotide on growth of A549 lung adenocarcinoma cell line apoptosis.docVIP

 PAGE \* MERGEFORMAT 10 Liposome-mediated VEGFC antisense oligonucleotide on growth of A549 lung adenocarcinoma cell line apoptosis Of: Guo Xufeng Qian Xu Zhonghua Chen Yongbing Yue YANG Wen-tao Xu Zhongheng [Abstract] Objective To investigate liposome-mediated vascular endothelial growth factor C (VEGF C antisense oligonucleotide on the growth of lung adenocarcinoma A549 cells, apoptosis. Methods A549 cells were divided into phosphate buffer the control group (PBS group), sense oligonucleotide group (SODN group) and antisense oligonucleotide transfection group (AODN group), each cell count method of counting cells, hematoxylin eosin (HE staining and cell morphology and distribution of A549 cells by flow cytometry analysis of apoptosis. application of Western blot detection of transfected A549 cells in each group after the VEGF C protein. Results VEGF C transfected antisense oligonucleotides after AODN degree of cell growth inhibition was significantly higher, the difference was statistically significant (P lt;0.01), slow growth and mitotic significantly reduced compared with the control group. AODN flow cytometry showed that cell apoptosis was significantly higher in the control group, the difference was statistically significant (P lt;0.01). Western blot showed that VEGF C antisense oligonucleotide could inhibit the VEGF C gene expression. Conclusion liposome-mediated antisense VEGF C can effectively interfere with VEGF C gene expression, and inhibit the growth of lung adenocarcinoma A549 cells and promote their apoptosis. [Keywords:] lung cancer, antisense oligonucleotides, apoptosis With non-small cell lung cancer (NSCLC) comprehensive treatment and individual treatment deepening the understanding and promotion of, NSCLC treatment has made great progress, but the current 5-year survival rate of NSCLC is still below 15%. The early stage of lung cancer can lead to lymph node metastasis is one of the main very poor prognosis. Our previous results h