Matrine induced lung cancer SK-MES-1 cells and its possible mechanism.doc

 PAGE \* MERGEFORMAT 12 Matrine induced lung cancer SK-MES-1 cells and its possible mechanism [Abstract] Objective: To investigate the matrine (MT induced lung cancer cell line SK-MES-1 and anti-tumor mechanism of apoptosis. Methods: Human lung squamous carcinoma cell line SK-MES-1, with different concentrations The MT treatment SK-MES-1 cells, MTT assay using MT on SK-MES-1 cell proliferation, apoptosis rate by flow cytometry and cell cycle, flow cytometry-related apoptosis protein Bcl-2, bax, caspase-3 expression. Results: MT inhibition of SK-MES-1 cell proliferation and induction of SK-MES-1 cell apoptosis in a dose and time dependent. Compared with the control group, bitter Participation after alkali treatment SK-MES-1 cells significantly increased the percentage of G0/G1 phase, S phase cells decreased. Bax and increased expression of capase-3, Bcl-2 was decreased. CONCLUSION: MT in vitro inhibition of human lung cancer cell line SK-MES-1 proliferation and induce apoptosis, which induced a significant relationship between dose and time may be related to inhibition of Bcl-2 activity, activation of bax and capase-3 related. [Keywords:] matrine lung squamous cell carcinoma cells SK-MES-1 Abstract: Objective: To explore the effects of matrine on the cell cycle and apoptosis in human lung squamous cell carcinoma SK-MES-1 cells and explore the possible mechanisms. Methods: The effect of matrine on cell proliferation was assessed using MTT assay. The cell cycle arrest and the apoptosis rate induced by matrine were determined with flow cytometry. The expression of caspase-3, Bcl-2 and Bax proteins were assayed by flow cytometry. Results: Matrine inhibited the proliferation of SK-MES-1 cells in a dose-and time-dependent manner. Compared with the control group, the matrine-treated cells showed increased cell percentage arrested in G0/G1 phase with decreased S-phase cells. After treatment of 48 hours, the apoptosis rate was increased. The expression of casp