Medium composition on callus proliferation and Carassius Fritillaria Alkaloids.doc

 PAGE \* MERGEFORMAT 12 Medium composition on callus proliferation and Carassius Fritillaria Alkaloids [Abstract] Objective To study the MS medium with a large number of elements, trace elements CARASSIUS Fritillaria callus growth and alkaloid content, selection was beneficial Penzer Fritillaria alkaloids in callus growth and accumulation of medium formula. method of orthogonal and single factor experimental design, and 40 d of Penzer Fritillaria growth of callus growth rate and alkaloid content as an indicator to evaluate the size of their impact factors. the result of various factors on the more Carassius Fritillaria Growth of injured tissue the size of the order of Pgt; Cagt; Kgt; N, on the order of alkaloid accumulation of Kgt; Ngt; Cagt; P, NO3-N content of the medium is high, is conducive to the accumulation of alkaloids, trace element deficiency are conducive to the growth of Carassius Fritillaria callus, lack of Cu, I lack the time is not conducive to the accumulation of alkaloids. Conclusion benefit Penzer Fritillaria callus growth and accumulation of alkaloids were the best combination : N3P1K3Ca2, N1P2K3Ca1, medium, large number of elements, trace elements CARASSIUS Fritillaria Alkaloids in callus growth and there is a certain extent. [Keywords:] Penzer Fritillaria alkaloids in callus growth Abstract: ObjectiveTo study the effect of large elements, trace elements of MS culture medium on the growth and alkaloid content of Fritillaria monantha Migo callus, and to select the culture medium which is advantageous in its growth and alkaloid accumulation.MethodsWith the growth rate and alkaloid content of the 40 days’ callus as indexes, single-factor and orthogonal experimental design was conducted to evaluate the impact of various factors.ResultsThe effect of various factors on the growth of the callus is Pgt; Cagt; Kgt; N, and on the alkaloids was Kgt; Ngt; Cagt; P; the alkaloid content was higher when the proportion of NO3-N was higher than NH +