Metabotropic glutamate receptor in diabetic neuropathic pain in rats the role of.doc

 PAGE \* MERGEFORMAT 13 Metabotropic glutamate receptor in diabetic neuropathic pain in rats the role of Of: Kechang Bin Huang Xiaoxia Luo Xiang-hong Li Qing Liu Juying 1 [Abstract] Objective To evaluate the metabotropic glutamate receptor (mGluR5) in rat neuropathic pain in diabetes. Methods 3 months old Wistar rats of either sex, weighing 180 ~ 220 g, a single intraperitoneal injection of streptozotocin streptozotocin (STZ) 65 mg / kg of diabetes rat model of neuropathic pain. model was successfully 64 rats were randomly divided into 2 groups (n = 32), diabetic neuropathic pain group (D group) and the mGluR5 antagonist dose group (M group), In another 32 rats served as normal control group (C group, n = 32). M group were injected with mGluR5 antagonists 20 mg / kg, 1 time / d, C, D group injected with saline. in each group were 2,4,6,8 weeks (T1 ~ 4) the end of 8 rats were randomly selected, measured using von Frey filaments after two-legged robot flinching response threshold (MWT), take L3 6 spinal cord, using immunohistochemistry and RT PCR detection of mRNA levels of mGluR5. The results with the C group, D group T1 ~ 4 ? MWT reduced, mGluR5 mRNA levels (P lt;0.05); with the D group, M group T1 ~ 4 ? MWT increased mGluR5 mRNA levels decreased (P lt;0.05). Conclusion mGluR5 activation in diabetic neuropathic pain in rats formed. [Keywords:] receptor; metabotropic glutamate; diabetic neuropathy; neuralgia [Abstract] Objective To evaluate the role played by mGluR5 in diabetic neuropathic pain (DNP) in rats. Methods 96 Wistar rats 3 months old weighing 180 ~ 220 g were randomly divided into 3 groups: group control (C); group DNP; group MPEP (selective mGluR5 inhibitor) + DNP. Diabetes mellitus (DM) was induced by intraperitoneal streptozocin 65 mg / kg. Blood glucose level was measured every week for 4 weeks. DM was confirmed by blood glucose levelgt; = 16.0 mmol / L in the 4th week. Mechanical paw withdrawal threshold to von Frey filament stimul