Metarhizium anisopliae acid trehalase gene sequence characteristics of.docVIP

 PAGE \* MERGEFORMAT 13 Metarhizium anisopliae acid trehalase gene sequence characteristics of [Abstract] Objective To obtain the acid trehalase gene (ATM1) and its sequence analysis. Methods primers designed according to the known sequence, PCR amplification of Metarhizium anisopliae ATM1 cDNA and DNA sequences, their deduced amino acid sequence based on Multiple sequence alignment and structure of local sequence alignment analysis. Results ATM1 cDNA sequence contains an open reading frame (ORF) 3 219 bp, 3 ‘untranslated region (3’UTR) 175 bp and 5’ untranslated region ( 5’UTR) 498 bp. concluded successfully ATM1 gene, identified by sequence analysis of 4 conserved functional sequences. ATM1 CQMal02 gene in the genome as a single copy. [Keywords:] fungi, trehalase, sequence alignment Trehalose is a non-reducing disaccharide, accounting for insect hemolymph carbohydrate 80% to 90% in insect physiology plays an important role in the blood, and its depletion may be lethal to the insects, the role of [1]. Metarhizium anisopliae (metarhizium anisopliae, Ma is a class of widely used insect pathogenic fungi in insect control plays an important role. Ma after the infection into the insect haemolymph trehalose into the growth of the main nutrients, secretion of Metarhizium anisopliae to Acid trehalase, effective degradation of trehalose in the haemolymph of insects. Thus acid trehalase in fungi, insects play an important role in pathogenesis [2]. Acidic trehalase present study fungi are only a few species of acid trehalase gene was cloned and sequenced [35]. The test isolation and cloning of acid trehalase Ma CQMal02 ATM1 gene, and full-sequence analysis, in order to build Ma CQMal02 ATM1 gene expression vector and interference carrier of acid trehalase for the further studies and modified by genetic engineering strains of Metarhizium anisopliae, to improve their insecticidal toxicity to provide a theoretical foundation and technical reserves. 1 Materials a