Microfluidic oscillatory flow rapid reverse transcription-polymerase chain reaction detection of tobacco mosaic virus.docVIP

 PAGE \* MERGEFORMAT 13 Microfluidic oscillatory flow rapid reverse transcription-polymerase chain reaction detection of tobacco mosaic virus Of: WANG Hai-ying Li, Yu Yuan Zhang bamboo shoots [Abstract] established the oscillatory flow based on capillary reverse transcription polymerase chain reaction (RT PCR) detection of microfluidic devices of tobacco mosaic virus (TMV) of the new method. According to TMV movement protein gene of a pair of PCR primers of coarse particles purified TMV direct RT PCR. with 0.025% bovine serum albumin (BSA) on the reaction capillary inner wall of static and dynamic passivation to improve the PCR efficiency. In this microfluidic device for RT PCR, The flow rate was 70 μL / min, the detection limit of 0.01 μg cDNA; can successfully within 17 min the purpose of 179 bp amplified DNA fragments. [Keywords:] Microfluidic, oscillatory flow, reverse transcription polymerase chain reaction, tobacco mosaic virus 1 Introduction Plants in the early onset of rapid and accurate plant virus detection is very important [1]. Anti-transcription polymerase chain reaction (RT PCR) technology because of its high sensitivity and specificity, has become a widely used biological detection method. RT PCR technique is easy and highly sensitive detection methods other combination, such as ECL [2 9], laser induced fluorescence [10, 13] and capillary electrophoresis [14] and other detection technology, its sensitivity to further increased. Conventional PCR generally has a large heat capacity, thermal cycling for a long time, energy consumption is high, and has a large volume. To overcome these drawbacks, some researchers have carried out PCR microfluidic technology [15]. At present, micro- flow control PCR in two forms: static and dynamic pool PCR continuous flow PCR. The former is the miniaturization of traditional PCR, the main heat capacity of the system is optimized to reduce heating and cooling time. continuous flow PCR is the PCR solu