Microdialysis technique in the study of brain tissue.docVIP

 PAGE \* MERGEFORMAT 15 Microdialysis technique in the study of brain tissue Author: Yin-Sheng Chen, Chuang Ming-hua, Jiang Su Microdialysis technique is the push-pull perfusion and dialysis technology, combined, and gradually perfecting a new type of biological sampling can be anesthesia or conscious use of organisms, especially suitable for deep tissue and vital organs in vivo biochemistry. Intracerebral microdialysis technique was first applied in 1972 at Yale University reported in the monkey brain microdialysis study, which began to be used after the microdialysis of the brain neurotransmitter changes, pharmacokinetics, monitoring, and a number of diseases in brain nerve Physiological changes in the research, has more than 30 years of history. In this paper, micro-dialysis technique in the study of brain tissue are reviewed. A microdialysis probe recovery of principle and determination of Microdialysis technique is based on principles of dialysis as a basis for in vivo sampling technique, is a non-equilibrium conditions, ie outflow of dialysis fluid under test compounds in concentrations below its probe membrane surrounding the concentration of the sample matrix, filling buried in the organization The micro-dialysis probe, the tissue under test compounds spread along the concentration gradient into the dialysate has been a continuous strip out, so as to achieve the purpose of sampling from the living tissue, by measuring the outflow of fluid in the dialysate concentrations of analytes to study the tissue levels of analytes. This is a dynamic continuous sampling method. Simply put, the principle of microdialysis is equivalent to in the organization has created a ‘capillary’, so that the concentration of compounds under the action of the poor spread into the ‘capillary’, and then with the liquid flow out of in vitro testing. Microdialysis technique can be the biggest advantage is that basically do not interfere with the normal physiological process in v