Microwave irradiation on rat hippocampus COXI IV gene expression.doc

 PAGE \* MERGEFORMAT 11 Microwave irradiation on rat hippocampus COXI IV gene expression [Keywords] microwave; hippocampus; cytochrome C oxidase; energy metabolism Abstract: Objective To study the microwave irradiation in rat hippocampus after cytochrome C oxidase (COX) subunits I, IV mRNA expression and enzyme activity, and to explore the energy of microwave irradiation on hippocampal metabolism mechanism. Methods RT-PCR to detect microwave radiation after the hippocampus COXI, IV mRNA level, using cytochrome C reduction method and high-performance liquid chromatography (HPIC) were used to detect microwave radiation after the hippocampus of cytochrome C oxidase activity and adenosine triphosphate (ATP), adenosine diphosphate (ADP), an adenosine monophosphate (AMP) levels. The results of microwave irradiation, the rat hippocampus cytochrome C oxidase activity and ATP were significantly reduced, and with COXI, IV mRNA expression was consistent. Conclusion microwave irradiation can affect the hippocampus COXI, IV mRNA expression and reduced enzyme activity, thereby causing the energy metabolism. Keywords:: microwave; hippocampus; cytochrome C oxidase; energy metabolism Effect of microwave exposure on COXI and COXIV mRNA expression in hippocampus of rats Abstract: Objective To study mRNA expression of cytochrome c oxidase (COX) subunit Ⅰ , Ⅳ and activity of cytochrome c oxidase in hippocampus of rats after microwave exposure, and to interpret the molecular mechanism of microwave exposure on the energy metabolism deficit in hippocampus of rats . Methods The expression of cytochrome c oxidase subunit Ⅰ and Ⅳ mRNA after microwave exposure was detected by RT-PCR.The content of adenosine triphosphate (ATP), adenosine diphosphate (ADP), adenosine monophosphate (AMP) and activity of cytochrome c oxidase in hippocampus were detected by high performance liquid chromatography (HPLC) and reduced cytochrome c methods respectively.Results Decreased activity of cytochro