Methylprednisolone on experimental allergic encephalomyelitis of rats SRp30c.docVIP

 PAGE \* MERGEFORMAT 15 Methylprednisolone on experimental allergic encephalomyelitis of rats SRp30c Of: Wei Chi Sheng, Wang Xiaohui, Hung Ming Fan, Su Quanxi, Yu Qingyun, Zanglin Quan, Pan snow Diao [Abstract] Objective To study the different doses of methylprednisolone (MP) role in experimental allergic encephalomyelitis (EAE) rat model, the splicing factor SRp30c expression and effect relationship between the occurrence of MP and hormone treatment the possible mechanism of resistance. Wistar rat EAE model was constructed, the EAE rats were divided into high-dose group, low dose group, model control group, respectively, through the tail vein injection of MP 100 mg / kg, 25 mg / kg, such as saline ; not made an alternative model rats as normal control group, such as saline for injection. 5d administration rats were killed after spinal cord tissues extracted from fresh RT PCR, detection SRp30c mRNA expression analysis and efficacy. Results SRp30c expression in all groups, large and small dose group SRp30c mRNA expression was significantly higher than the model group, normal control group, the difference was significant; different scores improved in rats Kono SRp30c mRNA expression of the difference between the value of its negative correlation (r = -0.583, P lt;0.05). Conclusion SRp30c MP expression and disease and not directly related to dose, but was negatively correlated with efficacy, suggesting that glucocorticoid sensitivity with the closely related decline, EAE model of the mechanism of hormone resistance occurs SRp30c play an important role. [Keywords:] methylprednisolone in experimental allergic encephalomyelitis glucocorticoid resistance SRp30c Abstract: Objective To investigate the expression of alternative splicing factor SRp30c and the therapeutic effect of methylprednisolone (MP) and the correlation between SRp30c and the therapeutic effect in experimental allergic encephalomyelitis (EAE) in rats after treatment with MP at different do