Midkine specific shRNA on gastric cancer cell proliferation and apoptosis BGC823.doc

 PAGE \* MERGEFORMAT 16 Midkine specific shRNA on gastric cancer cell proliferation and apoptosis BGC823 Of: Wang Qingling Huang Ya-Hong, Hong Liu, Hou Yayi [Abstract] Objective shRNA interference BGC823 midkine on gastric cancer cell proliferation and apoptosis. Midkine gene was constructed specific small interfering RNA plasmids, using Lipofectamine 2000 transfection BGC823 cells, the use of CCK-8 test cells proliferation, PI staining to detect cell apoptosis. Results The BGC823 transfected recombinant cell proliferation was significantly inhibited (P lt;0.01) cells to form colonies significantly decreased (P lt;0.01), and a clear hypodiploid peak occurred (P lt;0.05). Conclusion midkine gene for specific small interfering RNA plasmid can inhibit in vitro human gastric cancer cells BGC823 midkine expression, cell proliferation decreased, apoptosis increased midkine gene targeting therapy to certain experimental basis. [Keywords:] midkine; shRNA; gastric cancer cells; cell proliferation; cell apoptosis Abstract: Objective To investigate the effect of knockdown of midkine (MK) expression by shRNA on cell proliferation and apoptosis of human gastric carcinoma cell BGC823. Methods Specific shRNA plasmids to midkine were constructed, and were then transfected into BGC823 by lipofectamin 2000. The cell proliferation was evaluated by CCK-8 kit, and the apoptosis was determined by flow cytometric analysis. Results The recombinant plasmid expressing midkine shRNA effectively inhibited BGC823 cell proliferation (P lt;0.01) and decreased clone formation (P lt;0.01) and significantly promoted cell apoptosis with the emergence of sub-diploid peak (P lt;0.05). Conclusion shRNA plasmids targeting MK gene can inhibit the growth of human gastric cancer cells by attenuating cell proliferation and inducing apoptosis, which might provide experimental evidence for gene targeting therapy of MK. Keywords:: midkine; shRNA; gastric cancer cell; cell proliferation; apop