Mitochondrial pathway induced by deoxycholic acid in the esophageal mucosal epithelial cells apoptosis.doc

 PAGE \* MERGEFORMAT 18 Mitochondrial pathway induced by deoxycholic acid in the esophageal mucosal epithelial cells apoptosis Of: Wujian Tao, Xu Jiao, Gong Jun, Wang Jinhai, Dong Lei [Abstract] Objective To observe the mitochondrial pathway in the deoxycholic acid (deoxycholate, DCA) induced cultured human normal esophageal mucosal epithelial cells apoptosis. Methods serum-free medium for keratinocytes cultured human normal esophageal mucosal epithelial cells , Rh123 by flow cytometry and / PI fluorescence intensity of mitochondrial transmembrane potential (m) changes; Western blot detection of cytochrome c (Cyt.c), Caspase 3 and poly (ADP ribose) polymerase (PARP) inhibitor on expression of apoptosis and activation of Caspase 3. Results of esophageal epithelial cells with 100mol / L of DCA treatment, PI-negative but low Rh123 stained cells in the control group (1.8 + -0.6)%, 48h and (30.5 + -1.7)%; with 200mol / L of DCA treatment, PI-negative but low Rh123 stained cells up to 36h (43.1 + -0.2)% (P lt;0.05). PI-negative However, Rh123 low cells began to appear, and gradually increase, DCA induced apoptosis and mitochondrial membrane potential decreased, indicating that the apoptotic cells in gradually increased. Cyt.c and activation of Caspase 3 antibody shows that with the decline of mitochondrial m , Cyt.c, Caspase 3 activation of plasminogen has also been cut, Casapse 3 substrate PARP, activation of the degradation occurred. Caspase 3 inhibitor DEVD FMK partially inhibited the DCA induced apoptosis of esophageal epithelial cells, but mitochondria decline in membrane potential is not affected. Conclusion DCA induced apoptosis of esophageal epithelial cells induced by the mitochondrial pathway, mitochondria, Caspase 3, PARP activation to participate in the esophageal mucosa, DCA induced apoptosis. [Keywords:] deoxycholic acid; esophageal mucosal epithelial cells; mitochondrial pathway; apoptosis ABSTRACT: Objective To study the role of mitochondr