mitoKATP channel openers on isolated rabbit heart ischemia-reperfusion injury in rats.doc

 PAGE \* MERGEFORMAT 9 mitoKATP channel openers on isolated rabbit heart ischemia-reperfusion injury in rats [Abstract] Objective To observe the different types of ATP-sensitive potassium channel opener on potassium to stop he jumped off the rabbit heart ischemia-reperfusion injury in rats and to explore the possible protection mechanisms. Methods The in vitro experimental model of rabbit hearts Langendorff perfusion, 40 isolated rabbit hearts were randomly divided into five groups (n = 8): control group (C group), Pinacidil group (P group), Diazoxide group (D group), 5 -HD Pinacidil group (HP group) ,5-HD Diazoxide Group (HD Group). Isolated rabbit hearts 4 ℃ standard St.Thomas cardioplegia (K 16mmol / L) to cardiac arrest, 45min reperfusion after 20min, before the infusion of drugs in cardiac arrest 15min. Contrast observation Pinacidil, Diazoxide and 5-HD in combination with the cardiac function parameters, coronary blood flow and myocardial reperfusion at the end of adenosine, malondialdehyde (MDA) content, superoxide dismutase (SOD) activity and myocardial enzyme changes. Results P group, D group, HP group after reperfusion re-jump time, the recovery rate of cardiac function, myocardial energy preservation, SOD activity were significantly higher than C group, enzymes, MDA content was significantly lower than C group, HP group Heart functional recovery, energy preservation worse than P group, enzymes, MDA content higher than the P group. Conclusion muscle fiber membrane-type (sarcKATP channels) and mitochondrial-type (mitoKATP channel) KATP channels participate in myocardial ischemia-reperfusion injury in rats, in which mitoKATP channels play a dominant role. [Keywords:] mitoKATP channel; ischemia-reperfusion injury; isolated rabbit; Heart [Abstract] Objective To investigate the protective effects of different ATP-sensitive potassium (KATP) channel opens, Pinacidil and Diazoxide, on myocardium injury in isolated rabbit hearts caused by ischemia /