MMSA1 in multiple myeloma secondary structure prediction of antigen and antibody preparation.docVIP

 PAGE \* MERGEFORMAT 15 MMSA1 in multiple myeloma secondary structure prediction of antigen and antibody preparation Of: Zhou Fu-ling, Zhang Wanggang, Wang Bai Yan, Zhang Pengyu, ancient Liufang, ZHANG Wen-juan, Meng Xin, Tian Wei [Abstract] Objective To study the use of bioinformatics human multiple myeloma MMSA 1 (GenBank: AY952881) gene expression in protein secondary structure and B cell epitopes and polyclonal antibodies. Methods DNAStar Protean software modules analysis and prediction of protein secondary structure MMSA 1 and B cell epitopes. using multi-channel simultaneous solid-phase synthesis of 15 automatic peptide synthesis system, the immune health of New Zealand adult rabbits, preparation of the corresponding polyclonal antibodies. Results MMSA 1 proteins contain more -sheet and the corner structure. comprehensive flexibility, hydrophilicity, antigenicity, surface probability, secondary structure, and experimental ease the difficulty of coupling factors, selected MMSA 1 protein sequence of 318 ~ 333 as the antigen bit, that SSSLLPQSPAPTEHL, 15 peptide synthesis products by HPLC purification and mass spectrometry, purity 98.65%, the sequence is correct. immune rabbit serum antibodies obtained by affinity chromatography, ELISA test titer: 1:6000. Conclusion This study is based on human MMSA 1 epitope immunotherapy means provides a theoretical basis. [Keywords:] multiple myeloma; MMSA 1; secondary structure; epitope; polyclonal antibody ABSTRACT: Objective Prediction was made for a novel antigen (multiple myeloma special antigen) MMSA 1 in human multiple myeloma by using bioinformatics. We predicted the secondary structure and B cell epitopes, and elicited specific antibody using peptide microspheres and adjuvants. Methods The secondary structure and B cell epitopes of MMSA 1 protein were determined by graphic analysis using the Protean module (DNAstar Inc.). Antigenic peptides were synthesized as the antigen with Fmoc / PyBOP method. Adu