Mount Tai white flowers of high-quality total RNA Danshen root extraction and DDRT-PCR detection of.doc

 PAGE \* MERGEFORMAT 14 Mount Tai white flowers of high-quality total RNA Danshen root extraction and DDRT-PCR detection of Author: Wang bodybuilding, Ren-Jiu, Miao Miao, Yan-Ling Li, Hao Gang-Ping [Abstract] Objective rich in polyphenols and polysaccharides in the root of Salvia Taishan white flowers isolated high quality RNA. Comparison of the five kinds of RNA extraction methods, a sense of order to CTAB method based on a complete separation of high-quality RNA, simple and rapid method. The results using this method from the roots of Mount Tai white flowers Salvia purity of isolated RNA is: A260/A280 for 1.87, A260/A230 was 2.11, the yield is 284.62μ g / g. FW of the root, clear electrophoretic bands, RNA integrity of the good. RNA derived from this method can be directly used for cDNA library construction and other molecular biology experiments. Conclusion The technology can be effectively from the Taishan Mountain is rich in polyphenols and polysaccharides isolated from the root of white flowers Salvia high-quality RNA, as in the Shandong-specific medicine to lay the foundation for molecular biology. [Keywords:] Mount Tai white flowers Salvia; roots; RNA extraction Abstract: ObjectiveTo explore the high quality total RNA isolation method from root of Taishan Salvia miltiorrhiza Bge. F.alba CY WU et HW Li containing much polyphenols and amylose.MethodsSimple and rapid procedure for high-quality total RNA isolation modified from CTAB based method was obtained by comparing five RNA isolation methods. ResultsA260/A280 and A260/A230 of prepared RNA were respectively 1.87 and 2.11, and RNA amount were 284.62μ g / g fresh weight of root. The results of electrophoresis showed that RNA bands were clear and showed no degradation. This high quality RNA could meet the demands of molecular analysis, such as cDNA library construction.ConclusionThis modified technique based on CTAB method may be efficient and reliable in isolating high quality RNA from Taishan Salvia