Moxibustion rat model of aging degree of peripheral blood lymphocyte DNA damage effects of experimental study.doc

 PAGE \* MERGEFORMAT 12 Moxibustion rat model of aging degree of peripheral blood lymphocyte DNA damage effects of experimental study 【Abstract】 Objective: To explore the moxibustion “Zusanli” anti-aging mechanism. Methods: ozone aging mice “Zusanli” Point implementation of the moxibustion treatment, with the comet assay (Comet Assay) test in mice peripheral blood lymphocyte DNA damage level. Results: Moxibustion mouse tail DNA, peripheral blood lymphocytes was significantly decreased (P lt;0.05), tail length significantly shortened (P lt;0.05), the difference was statistically significant. Conclusion: Moxibustion able to enhance the body’s own ability of anti-free radical oxidative damage, reduce the level of lymphocyte DNA damage, so as to achieve anti-aging purposes. Keywords: lymphocyte DNA oxidative damage in aging moxibustion mice Zusanli In the aging study found that increased with age there are different levels of DNA damage and damage accumulation of various phenomena [1,2]. In vivo, leading to DNA damage in the material, mostly in the widespread metabolic processes of biological free radicals. Of its redox reactions induced by excessive, long-term results of random poisoning is caused by one of the causes of biological aging [3]. The decline in immune system function, one of the main features of aging, acupuncture can effectively improve the machine within the organs of the immune activity of antioxidant enzymes, reducing lipid peroxidation [4], but at the same time, can play on the immune cells the protective effect of DNA within the current limited involvement. In this study, experimental observation of the comet moxibustion “Zusanli” on ozone (O3) aging model rat peripheral blood lymphocytes DNA damage extent, and thus to explore the possibility of anti-aging mechanism of moxibustion. 1 Materials and methods 1.1 experimental animals, the main drug reagents and equipment to health, three-month-old Kunming male