Multicolor fluorescence in situ hybridization detection of acute lymphoblastic leukemia complex chromosomal abnormalities.doc

 PAGE \* MERGEFORMAT 16 Multicolor fluorescence in situ hybridization detection of acute lymphoblastic leukemia complex chromosomal abnormalities Author: Jian-Yong Li, horsepower, Xiao Bing, Jin-Lan Pan, Hai-Rong Qiu, Wu Yafang, Wen Bing Zhao, Yong-Quan Xue Abstract In this study the establishment of multi-color fluorescence in situ hybridization (M-FISH) technology platform to explore the detection of acute lymphoblastic leukemia (ALL) complex chromosomal aberrations in the application. Combined with conventional cytogenetic methods, and M-FISH Analysis of 5 cases with complex chromosomal abnormalities in patients with ALL. The results show that: M-FISH confirmed the original anomaly t (9; 22), t (1; 19) and t (y; 1), also found a new abnormal der (1) (1 ∷ 3 ∷ 7 ), der (6) t (6; 9) (q?; p13), der (1) t (1; 11), der (12) t (1; 12), der (3) t (3; 5 ), der (2) t (2; 16), der (9) (9 ∷ 18 ∷ 7) and der (7) (9 ∷ 18 ∷ 7), and correct the original error analysis, in which der (9 ) (9 ∷ 18 ∷ 7) and der (7) (9 ∷ 18 ∷ 7) for the world’s first reported. Conclusion: M-FISH in the detection of complex chromosomal ALL of the application prospect is to carry out precise karyotype analysis of advanced means indispensable. Keywords: multi-color fluorescence in situ hybridization Detection of the Complex Chromosomal Aberrations in Acute Lymphoblastic Leukemia by Means of Multiplex Fluorescence in situ Hybridization Abstract This study was aimed to establish the technique of multiplex fluorescence in situ hybridization (M-FISH) and to explore its usefulness in detection of complex chromosomal aberrations (CCAs) in acute lymphoblastic leukemia (ALL). Five ALL patients with CCAs were analyzed by combining the techniques of conventional cytogenetics (CC) and M-FISH. The results demonstrated that M-FISH confirmed the aberrations previously detected by CC, such as t (9; 22), t (1; 19) and t (y; 1 ), and revealed new abnormalities as der (1) (1 ∷ 3 ∷