MPTP-induced mouse model of Parkinsons disease-related brain areas-term expression of iNOS.doc

 PAGE \* MERGEFORMAT 7 MPTP-induced mouse model of Parkinson’s disease-related brain areas-term expression of iNOS Authors: Dong Yulin, Zhang Zhijun, Liu Juan, Yu Xiaoyan, Ni Heng Jian [Abstract] Objective: To observe the 1 - methyl -4 - phenyl -1,2,3,6 - tetrahydropyridine (MPTP)-induced PD model of acute injury in mice striatum (CPu), substantia nigra pars compacta (SNpc), frontal cortex association area (FrA) and ventral tegmental area (VTA) in the iNOS gene and protein expression changes of schedule. Methods: RT-PCR and Western blot methods on MPTP-induced PD model mice brain regions related to iNOS gene and protein expression were detected. Results: MPTP-induced PD model mouse SNpc and CPu brain iNOS gene and protein in various time points after administration were significantly expressed, of which 1 d is the expression of the peak; VTA and FrA brain iNOS gene and protein in Expression of SNpc and CPu brain areas did not express significant. Conclusion: The increased expression of iNOS may be SNpc and VTA brain DA neurons and the CPu and FrA brain regions peripheral DA neuron damage played a role, resulting in reduction of DA neuron degeneration and the occurrence of peripheral . [Keywords:] Parkinson’s disease; 1 - methyl -4 - phenyl -1,2,3,6 - tetrahydropyridine; inducible nitric oxide synthase; mice [Abstract] Objective: To observe the time-course expression of iNOS mRNA and protein in CPu, SNpc, FrA and VTA of the MPTP model of PD. Methods: Semi-quantitative RT-PCR and Western blot methods were used to detect the expressions of iNOS mRNA and proteins in these four regions. Results: The expressions of iNOS mRNA and proteins in CPu and SNpc increased significantly at several time points in the MPTP model of PD, and a peak was observed on day1.The expressions of iNOS mRNA and proteins in VTA and FrA increased less than those in CPu and SNpc. Conclusion: The increase in iNOS expression may damage the dopaminergic neurons in SNpc and VTA and the dopamin