Multidrug resistance-associated protein expression and HCC3 multidrug resistance.doc

 PAGE \* MERGEFORMAT 12 Multidrug resistance-associated protein expression and HCC3 multidrug resistance Of: Guo Xiaolin Jiang Yaqiu Jinqing Long [Abstract] Objective To investigate the multidrug resistance-associated protein 3 (MRP 3 expression and multidrug resistance in hepatocellular carcinoma. Methods RT PCR method for detection of normal liver cell L 02, liver cancer and liver cancer cells BEL resistance cell HepG2 / ADM the differences in the expression of MRP 3 mRNA, and then MRP 3 and sense oligonucleotide antisense fragments were transfected with liposome HepG2/ADM in resistant cells into liver cancer, use of MTT, flow cytometry and RT PCR detection of transfected cells with adriamycin (ADM fluorescence intensity, resistance index and the expression of MRP 3 mRNA. HepG2/ADM result in liver cancer cells resistant MRP 3 mRNA expression was significantly higher than that of normal liver cell L 02 and liver cancer cells BEL (P lt;0.05.MRP 3 antisense transfected into the resistant cells, can significantly reduce the resistance index of intracellular ADM, ADM increased intracellular concentration (P lt;0.05, the intracellular expression of MRP 3 mRNA than non-transfected cells decreased by 62.5% (P lt;0.05. Conclusions The high expression of MRP 3 may lead to liver cancer one of the mechanisms of multidrug resistance. [Keywords:] liver cancer, multidrug resistance, multidrug resistance associated protein 3 [Abstract] Objective To explore the relationship between the expression of multi drug resistance relative protein 3 (MRP 3) and liver cancer multi drug resistance. Methods The expression of MRP 3 in normal hepatocyte L 02, hepatocarcinoma cell BEL and hepatocarcinoma multi drug resistance cell line HepG2/ADM were detected by RT PCR. Anti and oligonucleotide of MRP 3 were transfected by lipidosome into HepG2/ADM, intra cellular doxorubicin (ADM) fluorescence intensity, resistance index, and the expression of MRP 3 mRNA were detected by MTT, fl