Multiple myeloma-associated antigen immunological screening and bioinformatics analysis.doc

 PAGE \* MERGEFORMAT 24 Multiple myeloma-associated antigen immunological screening and bioinformatics analysis Authors: Zhou Fu-ling, Zhang, Wang Gang, Chen Gang, Zhao Wan Hong, Cao Xing-mei, Chen Yin-Xia Tian Wei, Su-Hu Liu, WU Ming-xia, Liu Ming Abstract In this study, “cDNA expression library of the serological analysis (serological analysis of cDNA expression library, SEREX)” Screening of myeloma HMy2 cell cDNA expression library. Will receive all of the 30 positive clones were sequenced and compared to BLAST analysis of homologous sequences. The results showed that: access to known genes, 6, myeloma tumor antigens associated novel gene 12. The partial sequence of spliced EST, 6 known genes, such as the ring finger protein 167, KLF10 factor, TPT1 protein, p02 protein, cDNA FLJ46859 fis, DNMT1 methyltransferase enzymes, and along with other tumor formation, development and prognosis of certain relationship. The use of bioinformatics for the new gene structure and function of a preliminary analysis and forecast, which MMSA-3, MMSA-8 and MMSA-11 a complete coding region, encoding the protein length, respectively 215,160 and 122 amino acids; In addition to MMSA -1 may be located in sex chromosomes, the rest are likely located in autosomal; MMSA-4 and control the transcription of the tumor protein highly similar, MMSA-5 may be involved in phagocytosis, MMSA-7 may cause NF-κB inactivation, MMSA -12 may lymphocyte cytosolic protein. CrELISA preliminary analysis shows that, MMSA-3 and MMSA-7 and other new gene-specific higher. Conclusion: This study of the screening and identification of tumor antigens can be used for multiple myeloma in the early immunological diagnosis, residual disease monitoring, prognosis and tumor vaccine preparation and other research. Keywords: Multiple myeloma Immunological Screening for Multiple Myeloma-Associated Antigens and Their Bioinformatics Analysis Abstract This study was aimed to screen