Muscle tissue engineering scaffold to cells and human amniotic epithelial cells of the compatibility.doc

 PAGE \* MERGEFORMAT 14 Muscle tissue engineering scaffold to cells and human amniotic epithelial cells of the compatibility Study: Liu Ying Meng Xiaoting Xue Hui Yin Di Chen Dong Liu Jiamei [Abstract] Objective To make the muscle cells to tissue engineering, and testing with the biocompatibility of human amniotic epithelial cells. Methods SDS with TNT and chemical extraction methods to produce muscle cells engineering support, to observe the structure of frozen section. the kinds of human amniotic epithelial cells into the scaffold after 7 d culture, detected by immunohistochemistry amniotic epithelial cell proliferation activity, NT 3 and BDNF expression, scanning electron microscopy of ultrastructure. The results support the complete removal of cells, the main structure of parallel tubular structures. main component of the extracellular matrix of elastic fibers and collagen fibers intact. amniotic epithelial cell proliferation in the stent there and showed NT 3, BDNF immunoreactivity positive. scanning electron microscopy, amniotic epithelial cells were uniformly distributed in the scaffold, growth is good. Conclusions The production of the muscle cells to tissue engineering, human amniotic epithelial cells with good compatibility. [Keywords:] to the muscle cells; human amniotic epithelial cells; biocompatibility [Abstract] Objective To produce the acellular muscle as biomaterial scaffold and to observe its biocompatibility with the human amniotic epithelial cell. Methods The acellular muscle scaffolds were made by extraction with 3% Triton X 100 and 1% sodium dodecyl sulfate. The histological structure of acellular muscle was observed in frozen section. Then the cultured human amniotic cells were implanted into acellular muscle scaffolds. After cultured 1 w, the scaffolds with cells were sectioned, and BrdU, NT 3 and BDNF immunohistochemical staining were performed to observe the proliferating capacity and the expressions of NT 3 and BDNF of amn