Multi-drug resistant Escherichia coli 16S rRNA methylase aminoglycoside-modifying enzyme genes.doc

 PAGE \* MERGEFORMAT 5 Multi-drug resistant Escherichia coli 16S rRNA methylase aminoglycoside-modifying enzyme genes [Abstract] Objective To understand the multi-drug-resistant Escherichia coli (ECO) aminoglycoside resistance mechanisms. Methods PCR detection of 20 multi-resistant ECO bacteria 11 kinds of 16S rRNA methylase, and aminoglycoside-modifying enzyme genes. Results 1 strain detected in 16S rRNA methylase genes (5.0%), and for a new subtype emerges; 16 detected in aminoglycoside-modifying enzyme gene (80.0%). 15, the measured strains rmtB gene sequences translated into amino acid sequence and the United States nucleic acid library (GenBank) has been logged rmtB amino acids is different, to a new subtype. Conclusions The ECO multi-drug resistant bacteria and the production of aminoglycoside resistance mechanisms and the 16S rRNA methylase production of aminoglycoside-modifying enzymes associated. ECO multi-drug resistant bacteria, there are new aminoglycoside resistance mechanisms. [Keywords:] Escherichia coli; 16S rRNA methylase; aminoglycoside-modifying enzymes ABSTRACT Objective To investigate mechanism of aminoglycoside resistance in multidrug  resistant Escherichia coli. Methods The genes of 11 kinds 16S rRNA methylase and aminoglycoside  modifying enzymes were detected by polymerase chain reaction (PCR). Results In 20 strains, 1 strain carried 16SrRNA methylase gene (rmtB), 16 strains were positive for the genes of aminoglycoside  modifying enzymes. There were changes of aminoacids in the sequence of RmtB of No.15. A significant difference was found from the rest that landed in GenBank, which identified itself as new subtype. Conclusion The aminoglycoside resistance mechanism of the Escherichia coli was closely related with 16S rRNA methylase and aminoglycoside  modifying enzymes. The new mechanism of aminoglycoside resistance was discoved in multi  drug  resistant strains of Escherichia coli. KEY WORDS Escherichia coli; 16S rRNA