Mouse lung fibroblasts in the extracellular matrix scaffold in the growth characteristics.docVIP

 PAGE \* MERGEFORMAT 12 Mouse lung fibroblasts in the extracellular matrix scaffold in the growth characteristics Study: Step Xuefeng Ding Hao Yan Yulan Liu Yang [Abstract] Objective: To study the mouse lung fibroblasts (lung fibroblast, LF) in the extracellular matrix (extracellular matrix, ECM scaffold in the growth characteristics. METHODS: fibrinogen, laminin and fibronectin by a certain mixture ratio, in fresh rat plasma procoagulant effect, building a single blanket-like extracellular matrix scaffold, and also joined the transforming growth factor- (transforming growth factor , TGF ). the original passage cultured and purified rat LF stents, inverted microscope, immunofluorescence and Western blot LF mice was observed in the growth characteristics of three-dimensional culture. Results: The mouse LF and join in the ECM scaffold of the stent in the TGF cell proliferation, transformation and synthesis of collagen type ability were significantly stronger than normal monolayer culture. CONCLUSION: LF in the ECM scaffold in mice with good growth characteristics, for the LF in vitro interstitial pulmonary fibrosis provides a new approach. [Keywords:] lung fibroblasts, extracellular matrix, three-dimensional culture [Abstract] Objective: To investigate the biological characteristics of lung fibroblasts from rats which were seeded on ECM scaffold.Methods: Under the role of promoting coagulation of fresh rat plasma, three materials including fibrinogen, laminin and fibronectin each at a certain concentration were constructed to the monolayer ECM scaffold, on the surface of which purified lung fibroblasts were planted, and the TGF were added in the ECM scaffold respectively.The biologic characteristics of lung fibroblasts were observed and analyzed by means of inverted microscope, immunofluorescence chemistry and Western blot . Results: The biological characteristics of lung fibroblasts in both ECM scaffold and ECM scaffold with TGF demonstrated more